Newly identified cell type could be the key to restoring damaged
salivary glands
Scientists make fundamental advance in understanding salivary gland
biology

Date:
April 12, 2022
Source:
Scripps Research Institute
Summary:
Scientists have discovered a special type of cell that resides in
salivary glands and is likely crucial for oral health.



FULL STORY ========================================================================== Scientists at Scripps Research and the National Institute of Dental
and Craniofacial Research have discovered a special type of cell that
resides in salivary glands and is likely crucial for oral health.


==========================================================================
As the researchers described in Cell Reports on April 12, 2022, the new
type of salivary gland cell called "ionocyte" that works to maintain
healthy concentrations of charged molecules -- ions -- of potassium,
calcium, chlorine, and other electrolytes in saliva. The scientists also
found that this type of ionocyte secretes a key growth factor (fibroblast growth factor 10, or FGF10), suggesting that it has a further role in
the repair of salivary glands after injury.

"These are unique cells, and we hope that by studying them we can develop better treatments for the many medical conditions that affect salivary
glands and related glands such as tear glands," says study co-senior
author Helen Makarenkova, PhD, associate professor in the Department of Molecular Medicine at Scripps Research.

Salivary glands produce saliva, which makes it much easier for animals
to swallow food. Saliva also contains enzymes that assist in digestion, antibodies and other immune elements to protect against infection, and
finely tuned concentrations of different ions to maintain the overall
health of teeth and oral tissues. Salivary glands can be damaged by cancer-related radiation therapy in the head and neck region and other
medical conditions including autoimmune disorders.

"Each year, millions of Americans are diagnosed with dry mouth conditions, whose precise causes are often unclear," says study co-first author
Olivier Mauduit, PhD, a postdoctoral research associate in the Makarenkova
lab.

The team, together with co-senior author Matthew Hoffman, PhD, of the
National Institute of Dental and Craniofacial Research, focused first on
a growth factor protein called FGF10, which is important for the early development of salivary glands, and is suspected to have a maintenance
and repair function in adult salivary glands. The scientists' aim was
to discover the cell type that produces FGF10 in adult salivary glands.



==========================================================================
The team analyzed large single cell atlases of mouse gene activity, and isolated FGF10-expressing cells for in-depth gene expression analysis. In
this way, they found that while mesenchymal cells called fibroblasts
produce FGF10 in very young mice, a very different cell type -- a type of salivary duct- lining epithelial cell -- takes over production starting
in the second week of life.

The researchers showed that this FGF10-producing epithelial cell has
molecular markers indicating that it is an ionocyte, an evolutionarily
ancient cell type that maintains proper levels of ions and related
molecules in local tissues.

Among the products of this ionocyte, they observed, is the cystic fibrosis transmembrane conductance regulator protein (CFTR). This protein is best
known as the cause of the lung disease cystic fibrosis when it is absent
in lungs through inherited mutation. However, it is also known to have
an important role in salivary and tear glands, where its deficiency
contributes to a common, inflammatory, dry-mouth/eye syndrome called
Sjo"gren's syndrome. The identification of the cell type that produces
CFTR in the adult salivary gland might thus lead to better therapies
for this syndrome, the researchers said.

The researchers noted too that this newly identified ionocyte's
FGF10-producing function makes it unique among ionocytes.

"The fact that this cell is the producer of FGF10 in adult salivary
glands suggests that it could have a big role in gland maintenance
and repair after injury," says study co-author Vanessa Delcroix, PhD,
a postdoctoral researcher in the Makarenkova laboratory.

Although their initial analyses concerned mouse cells, the researchers
found evidence of very similar salivary gland ionocytes in a protein
atlas of human tissues.

The researchers now are following up with further studies, including
in human cells. Their hope is that a better understanding of how these FGF10-making ionocytes work in the adult salivary gland will pave the
way for effective therapies for conditions affecting salivary glands as
well as tear glands, given the many similarities between the two.

Support for the research included grants from the National Eye Institute (5R01EY026202, 5R01EY028983) and the National Institute of Dental and Craniofacial Research (R01DE031044).


========================================================================== Story Source: Materials provided by Scripps_Research_Institute. Note:
Content may be edited for style and length.


========================================================================== Journal Reference:
1. Olivier Mauduit et al. A mesenchymal to epithelial switch in Fgf10
expression specifies an evolutionary-conserved population
of ionocytes in salivary glands. Cell Reports, 2022 DOI:
10.1016/j.celrep.2022.110663 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2022/04/220412161613.htm

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