T cell response not critical for immune memory to SARS-CoV-2 or recovery
from COVID-19, study finds

Date:
July 27, 2021
Source:
American Society for Microbiology
Summary:
New research conducted in monkeys reveals that T cells are
not critical for the recovery of primates from acute COVID-19
infections.



FULL STORY ==========================================================================
New research conducted in monkeys reveals that T cells are not critical
for the recovery of primates from acute COVID-19 infections. T cell
depletion does not induce severe disease, and T cells do not account for
the natural resistance of rhesus macaques to severe COVID-19. Furthermore, strongly T cell-depleted macaques still develop potent memory responses
to a second infection.


==========================================================================
The findings, published in the mBio, an open-access journal of the
American Society for Microbiology, have implications for the development
of second- generation vaccines and therapeutics.

"We started this study early in the pandemic, trying to figure out how
to make a good model to study the disease in humans using animals. The
monkeys turned out to be more resistant to the disease than we expected,
so we wanted to try to figure out why that was and try to gain some
insights into the disease in humans as well," said lead study author Kim Hasenkrug, PhD, senior investigator in the Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy
and Infectious Diseases, National Institutes of Health, in Hamilton,
Montana. "We now know that the antibody response is the most critical
response for protection by vaccination, not the T cell response." In the
new study, the researchers used classic reagents known to deplete CD4+
and CD8+ T cells in rhesus macaques. While CD8+ T cells directly attack infected cells and kill them, CD4+ T cells are helper T cells that trigger
the immune response by recognizing pathogens and secreting cytokines,
small proteins, that signal other immune cells to act, including CD8+
T cells and antibody-producing B cells.

One week after depleting the macaques of CD4+ T cells, CD8+ T cells,
or both at the same time, the researchers infected the animals with
SARS-CoV-2. "We depleted, we infected them and then we continued the
depletions during the first week of infection to make sure the animals
were well depleted. Then we studied their blood to see how they were
responding in terms of their T cells and B cells," said Hasenkrug. For six weeks, the researchers studied nasal swabs and bronchoalveolar lavages to measure virus in the nose, mouth and lungs, and rectal swabs to see if the
gut was shedding virus. After six weeks, the researchers re-challenged
the monkeys with SARS-CoV-2 and they repeated their collection of virus
and blood samples, which allowed the researchers to evaluate immune memory responses. "If there is a memory response, you get a much quicker immune response and control of the virus. That is how vaccinations work. Once
your body has seen a viral pathogen, the next time it sees it, you can
get a much faster and stronger immune response," said Dr. Hasenkrug.

The researchers found that the monkeys were able to mount a good memory response against the virus regardless of T cell depletion. "We found
we got really good memory responses regardless of whether we depleted
T cells or not.

Basically, we found very strong virus neutralizing antibodies, and they
are the most important antibodies in controlling the infection. That
was unexpected by most immunologists, virologists and vaccinologists,"
said Hasenkrug.

"The other thing that happens during a memory response is that
antibodies mature, becoming, stronger and more potent at binding the
viral pathogen. We saw indications of this through what's called "class switching,," said Hasenkrug.

"Class switching" was also unexpected in these monkeys with depleted
T cells.

"We don't have a firm explanation as to why that happened, but we think
it involves some sort of compensatory response, which you can see in
our study.

For example, when we depleted CD8+ T cells, we saw stronger CD4+
T cell or B cells responses in some animals. When the animals are
missing something, they will try to make up for it by making more of
something else." Hasenkrug doesn't know why the T cells didn't turn
out to be more important, but it is probably a good thing that they are
not required, because then, people who fail to mount sufficient T cell responses still have opportunities to recover.

"This implies that the innate immune response is critical for initial
control of the virus, rather than the adaptive immune responses we
studied," said Hasenkrug.

========================================================================== Story Source: Materials provided by
American_Society_for_Microbiology. Note: Content may be edited for style
and length.


========================================================================== Journal Reference:
1. Kim J. Hasenkrug, Friederike Feldmann, Lara Myers, Mario
L. Santiago,
Kejun Guo, Bradley S. Barrett, Kaylee L. Mickens, Aaron
Carmody, Atsushi Okumura, Deepashri Rao, Madison M. Collins,
Ronald J. Messer, Jamie Lovaglio, Carl Shaia, Rebecca Rosenke,
Neeltje van Doremalen, Chad Clancy, Greg Saturday, Patrick Hanley,
Brian J. Smith, Kimberly Meade- White, W. Lesley Shupert, David
W. Hawman, Heinz Feldmann. Recovery from Acute SARS-CoV-2 Infection
and Development of Anamnestic Immune Responses in T Cell-Depleted
Rhesus Macaques. mBio, 2021; DOI: 10.1128/mBio.01503- 21 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2021/07/210727104711.htm

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