Fruit fly offers lessons in good taste
Study shows food choice decisions require taste input

Date:
July 27, 2021
Source:
University of California - Riverside
Summary:
The fruit fly has multiple taste organs throughout its body to
detect chemicals, called tastants, that signal whether a food is
palatable or harmful. It is still unclear, however, how individual
neurons in each taste organ act to control feeding. To explore this
question, a team used the fly pharynx as a model to study whether
taste information regulates sugar and amino acid consumption at
the cellular level.



FULL STORY ==========================================================================
What can the fruit fly teach us about taste and how chemicals cause
our taste buds to recognize sweet, sour, bitter, umami, and salty
tastes? Quite a lot, according to University of California, Riverside, researchers who have published a study exploring the insect's sense
of taste.


========================================================================== "Insect feeding behavior directly impacts humans in many ways, from
disease- carrying mosquitos that seek human blood to pests whose appetite
can wreak havoc on the agricultural sector," said Anupama Dahanukar,
an associate professor of molecular, cell and systems biology, who led
the study appearing in the Journal of Neuroscience. "How insect taste
neurons are organized and how they function is critical for a deeper understanding of their feeding behavior." The fruit fly has multiple
taste organs throughout its body to detect chemicals, called tastants,
that signal whether a food is palatable or harmful.

It is still unclear, however, how individual neurons in each taste organ
act to control feeding. To explore this question, Dahanukar's team used
the fly pharynx as a model to study whether taste information regulates
sugar and amino acid consumption at the cellular level.

Dahanukar explained animals rely heavily on the sense of taste to make
feeding decisions, such as consuming nutritive foods while avoiding
toxic ones.

"In mammals, taste information is encoded by specialized cells present in
taste buds of the tongue," she said. "Taste receptors expressed in these
cells can detect different chemicals. Molecular and functional differences
in receptors expressed in different cells allow recognition of different tastes, such as salty, sour, sweet, bitter, or umami." Several new
studies in flies indicate individual taste neurons can detect compounds belonging to more than one taste category, raising some questions about
the distinct behavioral roles of individual taste neurons. If many
classes of taste neurons are activated by sugar, for example, how does activation of just one class of taste neurons affect behavior?


========================================================================== Dahanukar's team answered this question by genetically engineering a fly
in which only a single defined class of pharyngeal neurons is active. The
team then tested this fly in different feeding experiments to understand
what the fly can or cannot do compared to animals that have all their
taste neurons intact.

"We found single-taste neurons are capable of responding and activating behavioral responses to more than one tastant category -- sweet and amino
acids in our study," said Yu-Chieh David Chen, the first author of the
research paper. "We also found that a single tastant category -- amino
acids in our study -- can activate multiple classes of taste neurons."
The team also tested flies that had no functional taste neurons. Such
flies were incapable of making any proper feeding decisions, no matter
the food choices -- whether these were two attractive stimuli, one
attractive and one aversive, or one nutritive and the other nonnutritive.

The researchers found food choice decisions cannot be made in the absence
of taste input; the latter is critical for ensuring appropriate food
choice and feeding behavior. Further, flies that had pharyngeal sweet
taste neurons as the only source of taste input were consistently able
to select more palatable food.

"Altogether, our results argue for the existence of a combinatorial
coding system, wherein multiple neurons coordinate the response to any
given tastant," Dahanukar said.



==========================================================================
The study is the first to directly test the impact of loss of all taste
neurons on behavioral responses to tastants of different categories. It
is also the first to test whether a single class of taste neurons is
sufficient for food choice and feeding behavior.

"Along with several other recent studies in the field, our work also
invites revisiting some established ideas about how insect taste is
organized," Dahanukar said. "Rather than encoding tastes as in mammals,
flies appear to encode some combination of valence -- attractive versus aversive -- and tastant identity." Her team anticipates that knowing
how taste neurons work in flies will facilitate insect studies of greater health or agricultural importance.

"We are building tools for asking the same sorts of questions in
mosquitoes," Dahanukar said. "Such studies could offer potential
targets for manipulating feeding behaviors of pests or disease vectors
in surveillance or control strategies." She acknowledged that her lab
has only evaluated a single taste neuron within the system it set up,
with many more remaining to be studied.

"We are interested in understanding what these neurons sense and how they
act, individually and as part of a group, to control parameters that lead
to either promotion or cessation of food intake," said Vaibhav Menon,
a graduate student in Dahanukar's lab and a co-author on the study.

The team plans to apply some of the same strategies to investigate how
feeding behavior is controlled in mosquitoes.

The study was supported by the Whitehall Foundation, National
Institutes of Health, National Institute of Food and Agriculture of
the U.S. Department of Agriculture, and UCR Agricultural Experimental
Station. Chen was a Howard Hughes Medical Institute International Student Research Fellow at UCR.

========================================================================== Story Source: Materials provided by
University_of_California_-_Riverside. Original written by Iqbal
Pittalwala. Note: Content may be edited for style and length.


========================================================================== Journal Reference:
1. Yu-Chieh David Chen, Vaibhav Menon, Ryan Matthew Joseph, Anupama
Arun
Dahanukar. Control of Sugar and Amino Acid Feeding via Pharyngeal
Taste Neurons. The Journal of Neuroscience, 2021; 41 (27): 5791 DOI:
10.1523/ JNEUROSCI.1794-20.2021 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2021/07/210727171549.htm

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