erik simpson <eastside.erik@gmail.com> wrote:
On 1/4/24 4:20 PM, *Hemidactylus* wrote:Apparently it got much worse away from official gov’t messaging channels:
I guess the Florida surgeon general goofball appointed by Desantis is in >>> the news for scare mongering about mRNA vaccines again. This is a bitHey, it's Florida.
outside my wheelhouse but I’ll try to deal with it. My incoherence follows…
Here’s FL Department of Health’s proclamation about the dangers of mRNA >>> vaccines:
https://www.floridahealth.gov/newsroom/2024/01/20240103-halt-use-covid19-mrna-vaccines.pr.html
“The Surgeon General outlined concerns regarding nucleic acid contaminants
in the approved Pfizer and Moderna COVID-19 mRNA vaccines, particularly in >>> the presence of lipid nanoparticle complexes, and Simian Virus 40 (SV40) >>> promoter/enhancer DNA. Lipid nanoparticles are an efficient vehicle for
delivery of the mRNA in the COVID-19 vaccines into human cells and may
therefore be an equally efficient vehicle for delivering contaminant DNA >>> into human cells. The presence of SV40 promoter/enhancer DNA may also pose >>> a unique and heightened risk of DNA integration into human cells.”
Quoting Ladapo: “The FDA’s response does not provide data or evidence that
the DNA integration assessments they recommended themselves have been
performed. Instead, they pointed to genotoxicity studies – which are
inadequate assessments for DNA integration risk. In addition, they
obfuscated the difference between the SV40 promoter/enhancer and SV40
proteins, two elements that are distinct.”
Here’s what he’s apparently talking about:
https://www.fda.gov/media/174875/download
“No SV40
proteins are encoded for or are present in the vaccines…No SV40 proteins >>> are encoded by the nucleotide sequences present in the mRNA vaccines. The >>> treatment of the
products with DNAase also fragments any residual DNA template that might be >>> present after other manufacturing
steps. Thus, as noted above, following manufacture of the mRNA COVID-19
vaccines, no DNA encoding SV40
proteins is present in the residual DNA remaining in the products.”
Are they talking past each other? The SV40 proteins would be encoded for by >>> the associated viral sequences, which wouldn’t be involved in the mRNA >>> vaccine manufacture process as the SV40 promoter/enhancer merely regulates >>> expression of a downstream genetic sequence right? It is good to know the >>> SV40 products themselves don’t appear. But what importance is the promoter
enhancer outside the viral context except as a tool, from what I think is >>> going on, to express some associated resistance marker gene? Isn’t this >>> early on in manufacture to indicate successful cell transfection (or
bacterial transformation) with an associated (or linked?) spike protein
encoding sequence? I’m totally going on guesswork based on what little I >>> could gather after this new news cycle erupted.
I wasn’t previously aware of the SV40 component used in manufacture, but >>> it’s not the virus itself. Whew!
This is superficial:
https://apnews.com/article/853343189368
“The European Medicines Agency, which regulates vaccines in European Union
nations, explained that “non-functional” fragments of SV40’s DNA sequence
are used as “starting material” in producing the vaccine.
But those materials are broken down and removed in the manufacturing
process. Trace amounts might still remain at “very low levels” in the final
product, the agency and others acknowledged, but they are well within
established safety guidelines.”
So the SV40 promoter/enhancer thingy is in the vaccine starter kit which is >>> latter mangled or shredded by DNAases?
And on elusive integration Paul Offit was quoted: “One, it’s very hard for
a DNA fragment to enter a cell, specifically its nucleus, where the DNA
resides,” Offit explained in a phone interview Monday. “Your cytoplasm --
the white egg part of your cell -- doesn’t like DNA and has a variety of >>> mechanisms to rid itself of DNA.”
“Two, for that fragment of DNA to be integrated into your DNA, you also >>> have to have enzymes that disrupt the DNA and allow you to insert that
fragment,” he continued. “That’s what gene therapy is all about, and that’s
what makes gene therapy so hard to do.”
One would need an integrase?
Not sure about the reputability of this site but it seems OK:
https://www.techarp.com/science/mrna-vaccines-contaminated-sv40/
“The SV40 promoter is a DNA sequence that was derived from the SV40 (Simian
Virus 40) virus, and is not the same thing as the SV40 virus DNA. Think of >>> it as a genetic tool obtained from the SV40 virus, just like how botulinum >>> toxin is a treatment derived from the bacteria, Clostridium botulinum.” >>>
That seems an ok analogy.
“Michael Imperiale, a molecular biologist at the University of Michigan >>> Medical School, explained that the SV40 promoter, on its own, can’t cause >>> cancer. The part of SV40 that’s potentially cancer-causing, known as the >>> T-antigen, isn’t present in the vaccine”
That’s reassuring. Phillip Buckhaults is quoted: “It’s [SV promoter] just
the volume knob that drives high level expression of anything put under its >>> control, which in this case is just an antibiotic resistance marker.”
So part of selecting cells/bacteria that were successfully
transfected/transformed?
Quoting Health Canada:
“Plasmids are an essential starting material for the production of mRNA >>> vaccines. During the downstream process in mRNA vaccine manufacturing, the >>> plasmid DNA is digested with enzymes to small fragments, and further
removed to a level of not more than 10 ng/human dose, which is in line with >>> the World Health Organization’s recommendation concerning residual DNA in >>> biological drugs. The DNA is digested with enzymes post-transcription.” >>>
Also from the website: “The presence of residual DNA itself does not mean >>> that they will integrate into our genome. For one thing – these are DNA >>> fragments that have been broken down by the DNase enzyme.”
Here’s more on the manufacturing process:
https://www.scientificamerican.com/article/no-covid-mrna-vaccines-wont-damage-your-dna/
“To make an mRNA vaccine against COVID, scientists start with circular >>> pieces of DNA called plasmids that contain a gene for the spike protein of >>> SARS-CoV-2, the virus that causes the disease. The plasmids are amplified >>> into billions of copies inside of bacteria, and chemicals are then added to >>> release them from the bacteria. Enzymes are used to cut the plasmids into >>> linear pieces of DNA that encode the spike protein, and a different enzyme >>> converts that DNA into mRNA. Another enzyme is added to chop any remaining >>> DNA into tiny harmless fragments.”
So if bacteria are used the process is called transformation instead of
transfection according to my molecular biology text? Still the scary SV40 >>> promoter is there for the antibiotic resistance marker to cull the bacteria >>> lacking the means to be productive?
There’s more going on than the SV40 but that’s what interested me today.
I also found this tangentially related patent:
https://patents.google.com/patent/US20050064547A1/en
https://www.joemygod.com/2024/01/joseph-ladapo-covid-vaccines-are-the-anti-christ/
https://twitter.com/EricKleefeld/status/1742970911207133424
“DeSantis’ Florida surgeon general Dr. Joseph Ladapo says covid mRNA vaccines “the Anti-Christ of all products… That’s so wrong, you know, it’s
just complete disrespect to the human genome and the importance of
protecting it and preserving it, and that is our connection to God.””
I am dumfounded how this guy got where he is. Oh Desantis…never mind!
Nando Ronteltap <nando_ronteltap@live.nl> wrote:
You're a complete moron, and your reference is obviously a completeHow is Dr Wilson a complete moron? Because he lacks subjectivity on DNA contaminants integrating into our precious body fluids? Do you know why I only drink rain water, distilled water, and pure grain alcohol Nando?
moron. Lacking any kind of judgment. And it is no use to argue with you,
except for you to learn how to make a personal judgment.
On Saturday, January 6, 2024 at 9:27:31 AM UTC-5, zen cycle wrote:
On 1/6/2024 12:44 AM, *Hemidactylus* wrote:Fluoridation.....
Nando Ronteltap <nando_r...@live.nl> wrote:Why, to avoid communist infiltration from foreign substances being
You're a complete moron, and your reference is obviously a completeHow is Dr Wilson a complete moron? Because he lacks subjectivity on DNA
moron. Lacking any kind of judgment. And it is no use to argue with you, >>>> except for you to learn how to make a personal judgment.
contaminants integrating into our precious body fluids? Do you know why I >>> only drink rain water, distilled water, and pure grain alcohol Nando?
introduced into our precious bodily fluids without our knowledge of
consent, of course. I won't go so far as to deny a women my essence,
however.
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