https://journals.asm.org/doi/10.1128/mbio.01623-24
ABSTRACT
As the closest living relatives of animals, choanoflagellates offer
insights into the ancestry of animal cell physiology. Here, we report
the isolation and characterization of a colonial choanoflagellate from
Mono Lake, California. The choanoflagellate forms large spherical
colonies that are an order of magnitude larger
than those formed by the closely related choanoflagellate Salpingoeca rosetta. In cultures maintained in the laboratory, the lumen of the
spherical colony is filled with a branched network of extracellular
matrix and colonized by bacteria, including diverse Gammaproteobacteria
and Alphaproteobacteria. We propose to erect Barroeca monosierra gen.
nov., sp. nov. Hake, Burkhardt, Richter, and King to accommodate this extremophile choanoflagellate. The physical association between
bacteria and B. monosierra in culture presents a new experimental model
for investigating interactions among bacteria and eukaryotes. Future
work will investigate the nature of these interactions in wild
populations and the mechanisms underpinning the colonization of B.
monosierra spheres by bacteria.
On 2024-08-23 19:59:29 +0000, erik simpson said:
https://journals.asm.org/doi/10.1128/mbio.01623-24
ABSTRACT
As the closest living relatives of animals, choanoflagellates offer
insights into the ancestry of animal cell physiology. Here, we report
the isolation and characterization of a colonial choanoflagellate from
Mono Lake, California. The choanoflagellate forms large spherical
colonies that are an order of magnitude larger
by volume, by surface area, by diameter? It makes a huge difference.
 than those formed by the closely related choanoflagellate Salpingoeca
rosetta. In cultures maintained in the laboratory, the lumen of the
spherical colony is filled with a branched network of extracellular
matrix and colonized by bacteria, including diverse
Gammaproteobacteria and Alphaproteobacteria. We propose to erect
Barroeca monosierra gen. nov., sp. nov. Hake, Burkhardt, Richter, and
King to accommodate this extremophile choanoflagellate. The physical
association between bacteria and B. monosierra in culture presents a
new experimental model for investigating interactions among bacteria
and eukaryotes. Future work will investigate the nature of these
interactions in wild populations and the mechanisms underpinning the
colonization of B. monosierra spheres by bacteria.
On 24/08/2024 10:54, Athel Cornish-Bowden wrote:
On 2024-08-23 19:59:29 +0000, erik simpson said:
https://journals.asm.org/doi/10.1128/mbio.01623-24
ABSTRACT
As the closest living relatives of animals, choanoflagellates offer
insights into the ancestry of animal cell physiology. Here, we report
the isolation and characterization of a colonial choanoflagellate from
Mono Lake, California. The choanoflagellate forms large spherical
colonies that are an order of magnitude larger
by volume, by surface area, by diameter? It makes a huge difference.
It's open access.
S. rosetta colonies are 10-15 micrometers in diameter. B. monosierra colonies are 10-120 micrometers in diameter. The respective modes are
12 and 26 micrometers in diameter. So the maximum is an order of
magnitude larger in diameter, and the mode an order of magnitude larger
in volume. The caption to figure 1F applies order of magnitude to the diameter, in which case the abstract should have included the words "up
to".
than those formed by the closely related choanoflagellate Salpingoeca
rosetta. In cultures maintained in the laboratory, the lumen of the
spherical colony is filled with a branched network of extracellular
matrix and colonized by bacteria, including diverse Gammaproteobacteria
and Alphaproteobacteria. We propose to erect Barroeca monosierra gen.
nov., sp. nov. Hake, Burkhardt, Richter, and King to accommodate this
extremophile choanoflagellate. The physical association between
bacteria and B. monosierra in culture presents a new experimental model
for investigating interactions among bacteria and eukaryotes. Future
work will investigate the nature of these interactions in wild
populations and the mechanisms underpinning the colonization of B.
monosierra spheres by bacteria.
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